Idan Yelin
Saltation-mutation and vice versa
Strains were transformed with a cold-adapted chaperonin either before or after batch-dilution transfers for 200 generations.
Idan Yelin
Saltation-mutation and vice versa
Strains were transformed with a cold-adapted chaperonin either before or after batch-dilution transfers for 200 generations.
Idan Yelin
Saltation-mutation and vice versa
Strains were transformed with a cold-adapted chaperonin either before or after batch-dilution transfers for 200 generations.
Idan Yelin
Saltation-mutation and vice versa
Strains were transformed with a cold-adapted chaperonin either before or after batch-dilution transfers for 200 generations.
Idan Yelin
Saltation-mutation and vice versa
Strains were transformed with a cold-adapted chaperonin either before or after batch-dilution transfers for 200 generations.
Idan Yelin
Saltation-mutation and vice versa
Strains were transformed with a cold-adapted chaperonin either before or after batch-dilution transfers for 200 generations.
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In Evolthon we measured the fitness of the strains under 4 environmental conditions.
In short, we have performed pooled competition experiments in 4 conditions:
1. The Challenge condition - cold temperature (15c/20c for bacteria/yeast)
2. Regular - regular temperature of 37c/30c for bacteria/yeast
3. colder - 8c for both bacteria and yeast
4. Osmotic - 20c/15c and 1.2M NaCl/1.2M sorbitol for bacteria/yeast
For each condition, we performed 4 pooled competition in each conditions, and sequence the barcode region of the strains to evaluate the relative fitness (raw data can be found here).
Results are summarized in the following figures.
* You can download strains key for the following figures from here.
Bacteria
Yeast
